EXPERIMENTAL RESEARCH OF THE POSSIBILITIES OF INFLUENCE ON HEALING SMALL INTESTINAL ANASTOMOSIS

  • M. Tymchenko
  • Yu. Ivanova
  • O. Klimova
Keywords: enteric anastomosis, anastomotic leakage, phototherapy, tissue growth factors, experiment.

Abstract

Abstract. A promising area of further research is the use of a combination of components of tissue growth factors in combination with low-intensity electromagnetic effects of light radiation.

Objective. Investigation of various methods of influence on the healing of sutures of the small intestinal anastomosis in conditions of peritonitis.

Materials and methods. The experiment was conducted on 20 white Wistar male rats weighing 180-250 g, the content, care and methods of experimental work with which corresponded to the observance of the International Principles of the European Convention on the Protection of Vertebrate Animals (Strasbourg, 1985)

Results. The research work reflects the results of an experimental study, the purpose of which was to develop a method of sealing the small intestinal anastomosis under peritonitis. The experiment was performed on 20 white Wistar male rats weighing about 250 grams. The possibilities of using physical (light of a certain wavelength) and biological (tissue growth factors) methods in the prevention and treatment of acute inflammatory reactions and stimulation of reparative processes are shown. It is noted that a promising direction for further research on methods for the prevention of insolvency of small bowel anastomoses is the use of electromagnetic effects of low intensity of the light spectrum in combination with the application of tissue growth factors.

Conclusion. A promising direction for further research is the use of a combination of sealing lines with the application of components of tissue growth factors in combination with a low-intensity electromagnetic effect of light radiation.

References

1. Galimov OV, Gil’manov AZh, Hanov VO, Birjukova EN, Ibragimov TR. Profilaktika nesostojatel’nosti anastomozov polyh organov zheludochno-kishechnogo trakta (jeksperimental’noe issledovanie). Hirurgija. Zhurnal im. N.I. Pirogova. 2008;10:27-31. DOI: 10.25881/BPNMSC.2018.13.15.010 [In Russian]
2. Poy̆da OI, Melnyk VM. Nespromozhnist shviv anastomoziv v khirurhii tovstoi kyshky. Ukraïnskyy̆ Zhurnal Khirurhiï. 2011;2 (11):243-7. [In Ukrainian]
3. Arikanoglu Z, Cetinkaya Z, Akbulut S, Ilhan YS, Aygen E, Basbug M, Ayten R, Girgin M, Ilhan N, Dagli F. The effect of different suture materials on the safety of colon anastomosis in an experimental peritonitis model. Eur. Rev. Med. Pharmacol. Sci. 2013;17(19):2587-93. PMID: 24142603
4. Kryvorotko IV. Profilaktyka nespromozhnosti anasto-moziv pislia kombinovanykh operatsii z pryvodu mistsevo-rozpovsiudzhenoho raku priamoi kyshky (eksperymentalno-klinichne doslidzhennia) [avtoreferat]. Kharkiv. 2011. 30 p. [In Ukrainian]
5. Kaminskij IV, Chemodanov EB. Kliniko-jeksperimental’noe obosnovanie primenenija precizionnogo kishechnogo shva u bol’nyh s vysokoj stepen’ju verojatnosti vozniknovenija posleoperacionnyh oslozhnenij. Ukraїns’kij zhurnal hіrurgії. 2014;1(24):86-93. Dostupno na: http://www.mif-ua.com [In Russian]
6. Vinnik JuS, Markelova NM, Kuznecov MN. K voprosu o primenenii biopolimerov dlja profilaktiki nesostojatel’nosti kishechnyh anastomozov. Hirurgicheskaja praktika. 2013;1:30-5. [In Russian]
7. Sukhodolia AI, Nazarchuk SA, Dmytriiev DV. Obgruntuvannia zastosuvannia ksenodermoimplantativ dlia profilaktyky nespromozhnosti kyshechnykh shviv, anastomoziv v onkokhvorykh z krytychnymy stanamy. Bil, zneboliuvannia i intensyvna terapiia. 2015;3:44-51. DOI: 10.25284/2519-2078.3(72).2015.84590 [In Ukrainian]
8. Huh YJ, Lee HJ, Kim TH, Choi YS, Park JH, Son YG, Suh YS, Kong SH, Yang HK. Efficacy of Assessing Intraoperative Bowel Perfusion with Near-Infrared Camera in Laparoscopic Gastric Cancer Surgery. J. Laparoendosc. Adv. Surg. Tech A. 2019;29(4):476-83. DOI: 10.1089/lap.2018.0263
9. Adas G, Percem A, Adas M, Kemik O, Arikan S, Ustek D, Cakiris A, Abaci N, Kemik AS, Kamali G, Karahan S, Akcakaya A, Karatepe O. VEGF-A and FGF gene therapy accelerate healing of ischemic colonic anastomoses (experimental study). Int. J. Surg. 2011;9(6):467-71. DOI: 10.1016/j.ijsu.2011.05.002
10. Grotenhuis N, Vd Toom HF, Kops N, Bayon Y, Deerenberg EB, Mulder IM, van Osch GJ, Lange JF, Bastiaansen-Jenniskens YM. In vitro model to study the biomaterial-dependent reaction of macrophages in an inflammatory environment. Br. J. Surg. 2014;101(8):983-92. DOI: 10.1002/bjs.9523
11. Landes LC, Drescher D, Tagkalos E, Grimminger PP, Thieme R, Jansen-Winkeln B, Lang H, Gockel I. Upregulation of VEGFR1 in a rat model of esophagogastric anastomotic healing. Acta Chir Belg. 2018;118(3):161-6. DOI: 10.1080/00015458.2017.1394673
12. Shapkin JuG, Chalyk JuV, Klimashevich VJu, Stekol’nikov NJu, Odnokozova JuS. Primenenie lazerov v abdominal’noj hirurgii. Bulletin of Medical Internet Conferences. 2013;3(8):1054. . [In Russian]
13. Lei Su, Martina BF, Shobhit A, Hiromi K, Robert DG, George BH, Daniel SE. Laser-induced tissue fluorescence in radiofrequency tissue-fusion characterization. J. Biomed. Opt. 2014;19(1):15007. DOI: 10.1117/1.JBO.19.1.015007
14. Tarik ZN, Nebil B, Ruksan A, Tamer C, Turgut N, Gokhan M, Mehmet H. Effects of a static magnetic field on wound healing: results in experimental rat colon anastomoses. Am. J. Surg. 2006;192(1):76-81. DOI: 10.1016/j.amjsurg.2006.01.024
15. European convention for the protection of vertebrate animals used for experimental and other scientific purposes. – Council of Europe, Strasbourg, 1986. – 53 p.
Published
2020-10-12
How to Cite
Tymchenko, M., Ivanova, Y., & Klimova, O. (2020). EXPERIMENTAL RESEARCH OF THE POSSIBILITIES OF INFLUENCE ON HEALING SMALL INTESTINAL ANASTOMOSIS. Kharkiv Surgical School, (4), 55-60. https://doi.org/10.37699/2308-7005.4.2020.10
Section
EXPERIMENTAL RESEARCHES